Expression of inflammatory markers in pig amnion after intraamniotic infection with nonpathogenic or enteropathogenic<Emphasis Type="Italic">Escherichia coli</Emphasis> |
| |
Authors: | Email author" target="_blank">A??plíchalováEmail author I??plíchal I?Trebichavsky H?Hojná |
| |
Institution: | Institute of Microbiology, Academy of Sciences of the Czech Republic, 549 22 Novy Hrádek, Czechia. splichalova@biomed.cas.cz |
| |
Abstract: | The pig amnion was in vivo intraamniotically infected with E. coli for 10 h at 80-85 d of gestation either with the nonpathogenic O86 strain or enteropathogenic O55 strain. TNF-alpha, IL-10, IL-1beta and IFN-gamma were determined in amniotic fluids by ELISA, the expression of cytokines and some other inflammatory markers was determined by immunohistochemistry. Intraamniotic infection induced high levels of TNF-alpha in amniotic fluids which correlated with bacterial virulence whereas IL-10 was induced only by O86. The IL-1beta level did not increase significantly and was expressed in all infected membranes. IFN-gamma was negligible or absent. TNF-alpha, IL-12p40, calprotectin, HSP65 and gp91phox were found by immunohistochemistry only in amnion membranes infected with the enteropathogenic strain 055. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|