Mycoplasma pneumoniae and/or Chlamydophila pneumoniae inoculation causing different aggravations in cholesterol-induced atherosclerosis in apoE KO male mice |
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Authors: | Sueli B Damy Maria L Higuchi Jorge Timenetsky Márcia M Reis Suely P Palomino Renata N Ikegami Fabiana P Santos Junko T Osaka Luiz P Figueiredo |
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Institution: | (1) Medical School, University of Sao Paulo/LIM 26, Sao Paulo, Brazil;(2) Heart Institute (InCor) of Clinical Hospital, University of Sao Paulo, Sao Paulo, Brazil;(3) Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil;(4) Adolfo Lutz Institute, Sao Paulo, Brazil |
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Abstract: | Background
Chamydophila pneumoniae (CP) and/or Mycoplasma pneumoniae (MP) are two bacteria detected in vulnerable atheromas. In this study we aimed to analyze whether CP and/or MP aggravates
atherosclerosis induced by cholesterol-enriched diet in C57BL/6 apoE KO male mice. Thirty male apoE KO mice aged eight weeks
fed by a diet containing 1% cholesterol until 32 weeks of age were divided into four groups: the first was inoculated with
CP (n = 7), the second with MP (n = 12), the third with both CP + MP (n = 5), and the fourth with saline (sham n = 6). The
animals were re-inoculated at 36 weeks of age, and sacrificed at 40 weeks of age. Two ascending aorta and one aortic arch
segments were sampled. In the most severely obstructed segment, vessel diameter, plaque height, percentage of luminal obstruction
and the degree of adventitial inflammation were analyzed. The plaque area/intimal surface ratio was obtained by measuring
all three segments. The adventitial inflammation was semiquantified (0 absent, 1 mild, 2 moderate, and 3 diffuse). |
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