MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR |
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Authors: | Alexey A Fushan and Dennis T Drayna |
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Institution: | (1) National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA |
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Abstract: | Background Multiple approaches for the site-directed mutagenesis (SDM) have been developed. However, only several of them are designed
for simultaneous introduction of multiple nucleotide alterations, and these are time consuming. In addition, many of the existing
multiple SDM methods have technical limitations associated with type and number of mutations that can be introduced, or are
technically demanding and require special chemical reagents. |
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Keywords: | |
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