Selective Permeation and Organic Extraction of Recombinant Green Fluorescent Protein (gfp uv ) from Escherichia coli |
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Authors: | Thereza Christina Vessoni Penna Marina Ishii |
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Affiliation: | 1. Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Science, University of S?o, Paulo, Brazil
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Abstract: | Background Transformed cells of Escherichia coli DH5-α with pGFPuv, induced by IPTG (isopropyl-β-d-thiogalactopyranoside), express the green fluorescent protein (gfp uv ) during growth phases. E. coli subjected to the combination of selective permeation by freezing/thawing/sonication cycles followed by the three-phase partitioning extraction (TPP) method were compared to the direct application of TPP to the same culture of E. coli on releasing gfp uv from the over-expressing cells. |
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