Structural analysis of hemicatenated DNA loops |
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| Authors: | Claire Gaillard Luda S Shlyakhtenko Yuri L Lyubchenko Fran?ois Strauss |
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| Affiliation: | (1) Institut Jacques Monod, 2 place Jussieu, 75251 Paris, 05, France;(2) Department of Biology, Arizona State University, Tempe, AZ, 85287-2701, U.S.A;(3) Department of Microbiology, Arizona State University, Tempe, AZ, 85287-2701, U.S.A |
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| Abstract: | Background We have previously isolated a stable alternative DNA structure, which was formed in vitro by reassociation of the strands of DNA fragments containing a 62 bp tract of the CA-microsatellite poly(CA)·poly(TG). In the model which was proposed for this structure the double helix is folded into a loop, the base of the loop consists of a DNA junction in which one of the strands of one duplex passes between the two strands of the other duplex, forming a DNA hemicatenane in a hemiknot structure. The hemiknot DNA structures obtained with long CA/TG inserts have been imaged by AFM allowing us to directly visualize the loops. |
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