A modification of Representational Difference Analysis, with application to the cloning of a candidate in the Reelin signalling pathway |
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Authors: | Anelia A Kuvbachieva and Andre M Goffinet |
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Affiliation: | (1) Neurobiology Unit 2853, Univ. Namur Med. Sch., 61, rue de Bruxelles, Namur, Belgium;(2) Developmental Genetics Unit, Univ. Louvain Med. School, Avenue E. Mounier, 73, Box 82, B1200 Brussels, Belgium |
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Abstract: | Background cDNA-RDA is one of the subtractive cloning techniques used to isolate differentially expressed genes between two complex cDNA populations. In the present study we present a modification of the protocol described by Hubank and Schatz. |
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