Construction and Physiological Analysis of a Xanthomonas Mutant To Examine the Role of the oxyR Gene in Oxidant-Induced Protection against Peroxide Killing |
| |
| Authors: | Skorn Mongkolsuk Rojana Sukchawalit Suvit Loprasert Wipa Praituan and Apichat Upaichit |
| |
| Institution: | Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok 10210,1. and Department of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Rd, Bangkok 10400,2. Thailand |
| |
| Abstract: | We constructed and characterized a Xanthomonas campestris pv. phaseoli oxyR mutant. The mutant was hypersensitive to H2O2 and menadione killing and had reduced aerobic plating efficiency. The oxidants’ induction of the catalase and ahpC genes was also abolished in the mutant. Analysis of the adaptive responses showed that hydrogen peroxide-induced protection against hydrogen peroxide was lost, while menadione-induced protection against hydrogen peroxide was retained in the oxyR mutant. These results show that X. campestris pv. phaseoli oxyR is essential to peroxide adaptation and revealed the existence of a novel superoxide-inducible peroxide protection system that is independent of OxyR. |
| |
| Keywords: | |
|
|
