高危型HPV 感染对宫颈病变组织IFN-r、IL-10表达的影响

目的：探讨高危型人乳头瘤病毒（HPV）感染对宫颈病变组织干扰素-r(IFN-r)、白细胞介素-10(IL-10)的影响。方法：收集我院2013 年1 月到2015 年1 月妇科门诊行宫颈活检患者150 例，根据病理检查结果，将患者分为观察组（宫颈癌33 例、CINⅠ期26 例、CINⅡ期28 例、CINⅢ期23 例）110 例，对照组（慢性宫颈炎患者）40 例。提取两组患者宫颈组织标本，检测观察组HPV-DNA、HPV 分型，及两组IFN-r、IL-10 蛋白及mRNA 表达，分析高危型HPV感染与宫颈病变组织IFN-r、IL-10 表达的关系。结果：观察组HPV-DNA阳性87 例（79.1%），其中HPV16 型47 例（42.7%）、HPV18 型20 例（18.2%）感染率最高；且HPV16、18 感染率在CINⅠ期、CINⅡ期、CINⅢ期以及宫颈癌组中的感染率差异均有统计学意义（P<0.05），Spearman相关性分析显示，HPV16、18 与宫颈病变程度均呈现正相关关系（r=0.896，0.786；均P<0.05）。IFN-r、IL-10 表达水平在CINⅠ期、CINⅡ期、CINⅢ期、宫颈癌、对照组表达水平差异均具有统计学意义（P<0.05），Spearman 相关性分析显示，IFN-r与宫颈病变程度呈现负相关关系（r=-0.567，P<0.05），IL-10 呈现正相关关系（r=0.678，P<0.05）。且HPV16、HPV18 感染率与IFN-r表达呈现负相关关系（rHPV16/18=-0.678，-0.675，均P<0.05），与IL-10 呈现正相关关系（rHPV16/18=0.582，0.778，均P<0.05）。结论：IFN-r表达随着宫颈病变加重或者HPV16、HPV18 感染率升高逐渐降低，IL-10 则逐渐升高。

Effect of High Risk HPV Infection on Expression of IFN-r and IL-10 inCervical Lesions

Objective:To investigate the effect of high risk HPV infection on the expression of IFN-r and IL-10 in cervical lesions.Methods:A total of 150 patients, who accepted cervical biopsy in gynecological clinic of Hunan Tumor Hospital from January2013 to January 2015,were collected and divided into observation group (n=110 : 33 cases of cervical cancer, 26 cases in CINI stage, 28cases in CINII stage,23 cases in CINIII stage) and control group (n= 40 cases of chronic cervicitis patients) by pathological examinationresults. The cervical tissue specimens of patients in the two groups were extracted; the HPV-DNA,HPV typing and the expression of ofIFN-,IL-10 protein and mRNA in the two groups were detected; the correlation of high risk HPV infection with the expression of IFN-rand IL-10 was analyzed.Results:87 patients (79.1%) were HPV-DNA positive in the observation group, among which the infection ratesof 47 cases of HPV16 type (42.7%) and 20 cases of HPV18 type (18.2%) were the highest; the differences of infection rates of HPV16and18 in CINI, CINII, CINIII stages and cervical cancer group were statistically significant (P<0.05), Spearman correlation analysisshowed that HPV16 and 18 were positively correlated with cervical lesions degree (r=0.896, 0.786; P<0.05). The expression levels ofIFN-rand IL-10 in CINI stage, CINII stage, CINIII stage, cervical cancer and the control group were statistically significant(P<0.05),spearman correlation analysis showed that IFN-rwas negatively correlated with the cervical lesions degree (r=-0.567, P<0.05), and waspositively correlated with IL-10 (r=0.678, P<0.05). And the HPV16, HPV18 infection rates were negatively correlated with expression ofIFN-r(rHPV16/18=-0.678, -0.675, P<0.05), and were positively correlated with IL-10 (rHPV16/18=0.582, 0.778, P<0.05).Conclusion: