Proteolytic inactivation of a pentafunctional enzyme conjugate: coordinate protection by the first substrate. |
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Authors: | A Vitto F H Gaertner |
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Institution: | University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences and Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830 USA |
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Abstract: | The pentafunctional enzyme conjugate of was exposed to trypsin, chymotrypsin, or a protease preparation from in the presence and absence of the first substrate, 3-deoxy-D--heptulosonate 7-phosphate. It was found that the first substrate coordinately protects all five activities from proteolytic inactivation, which indicates a conformational change induced by this compound. In addition, the data presented are consistent with the “domain” theory of conjugate structure. It is also argued that coordinate protection may be of physiological significance. |
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Keywords: | pentafluorophenylalanine Boc tert -butyloxycarbonyl |
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