Inhibitory effect of irradiation products of cyclic adenosine-monophosphate on the relaxing ability of smooth muscle preparations |
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Authors: | L. Schachinger U. Hagen H. Klöter Ch. Schippel M. Jaenicke |
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Affiliation: | (1) Abteilung für Strahlenbiologie, Institut für Biologie, Gesellschaft für Strahlen und Umweltforschung m.b.H. München, D-8042 Neuherberg, Federal Republic of Germany |
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Abstract: | Summary In a previous publication it has been shown that the radiation induced physiological inactivation of dibutyryl-cAMP was far more pronounced than the chemical modification (Schachinger et al. 1981). In this paper it will be shown, that irradiation of dib-cAMP in solution resulted in the formation of monobutyryl cAMP and other not yet completely identified hydroxylated derivatives as well as a decomposition of the purine structure. Moreover, irradiated dib-cAMP inhibited the physiological activity of not irradiated dib-cAMP on the smooth muscle. From the data an effectiveKm-value for dib-cAMP of 2.4 × 10–5 M was determined and an effectiveKI-value of 1.3 × 10–6 M was found for the irradiation products, i.e., a tenfold affinity of the latter compared to unirradiated dib-cAMP. The results are discussed with respect to a better understanding of dose-response curves for chemical and physiological inactivation. |
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