Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres |
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Authors: | Faten Zaibak Paul Bello Jennifer Kozlovski Duncan Crombie Haozhi Ang Mirella Dottori Robert Williamson |
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Affiliation: | (1) Department of Paediatrics, The University of Melbourne, Royal Children's Hospital, Victoria, 3052, Australia;(2) Murdoch Childrens Research Institute, Royal Children's Hospital, Victoria, 3052, Australia;(3) Stem Cell Sciences Ltd, Babraham Research Campus, Cambridge, CB22 3AT, UK;(4) O'Brien Institute, Melbourne, Victoria, 3065, Australia;(5) Centre for Neurosciences, Department of Pharmacology, The University of Melbourne, Parkville, Victoria, 3010, Australia |
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Abstract: | Background Human umbilical cord blood-derived unrestricted somatic stem cells (USSCs), which are capable of multilineage differentiation, are currently under investigation for a number of therapeutic applications. A major obstacle to their clinical use is the fact that in vitro expansion is still dependent upon fetal calf serum, which could be a source of pathogens. In this study, we investigate the capacity of three different stem cell culture media to support USSCs in serum-free conditions; HEScGRO™, PSM and USSC growth mediumACF. Our findings demonstrate that USSCs do not grow in HEScGRO™ or PSM, but we were able to isolate, proliferate and maintain multipotency of three USSC lines in USSC growth mediumACF. |
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