The effect of various ions on the stability of crystalline salivary amylase in solution |
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| Authors: | BROCKETT F P CONNELLY C C MUUS J |
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| Affiliation: | 1. Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children''s Hospital, Medical Faculty Heinrich-Heine University, Moorenstr. 5, 40225 Duesseldorf, Germany;2. Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi, Ghana;3. Agogo Presbyterian Hospital, Agogo, Ghana;4. Department of Immunohematology & Blood Transfusion/Department of Infectious Diseases, Leiden University, the Netherlands;5. School of Public Health, College of Health Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana;1. Department of Physiology, Michigan State University, East Lansing, MI 48824, USA;1. Dipartimento di Ingegneria dell’Energia, dei Sistemi, del Territorio e delle Costruzioni – Università di Pisa, Largo L. Lazzarino, Pisa 56126, Italy;2. Dipartimento di Ingegneria Civile e Industriale – Università di Pisa, Largo L. Lazzarino, Pisa 56126, Italy;3. TOSCOTEC SPA, Head of Energy & Environment Dep. – Viale Europa, 55012 Marlia- Lucca Italy |
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| Abstract: | The rate of inactivation of salivary amylase at 45 °C. and pH 8.0 may be varied from a half-time of 7 min. in a phosphate buffer (reagent-grade), up to 4200 min. in a recrystallized diethylbarbiturate buffer containing cyanide and chloride. The recrystallization and cyanide effect is related to heavy-metal impurities, the chloride effect to its role as an activator. |
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