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A general assay for restriction endonucleases and other DNA-modifying enzymes with plasmid substrates |
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| Authors: | I Barry Vipond Geoffrey S Baldwin Mark Oram Symon G Erskine Lois M Wentzell Mark D Szczelkun Timothy J Nobbs Stephen E Halford |
| Institution: | (1) Department of Biochemistry, Center for Molecular Recognition, University of Bristol, BS8 1TD Bristol, UK |
| Abstract: | A procedure for measuring the activities of enzymes that alter the covalent structure of DNA is described. The assay utilizes covalently closed circles of DNA as the substrate and yields quantitative data on the fraction of this DNA converted to both open-circle and linear forms. |
| Keywords: | DNA electrophoresis plasmid restriction enzyme |
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