The suppressor of cytokine signaling (SOCS)-7 interacts with the actin cytoskeleton through vinexin |
| |
Authors: | Martens Nele Wery Maxime Wang Ping Braet Filip Gertler Arieh Hooghe Robert Vandenhaute Jean Hooghe-Peters Elisabeth L |
| |
Affiliation: | Neuroendocrine Immunology Group, Pharmacology Department, Medical School, Free University of Brussels (VUB), 1090, Belgium. nmartens@farc.vub.ac.be |
| |
Abstract: | To understand the function of the suppressor of cytokine signaling (SOCS)-7, we have looked for proteins interacting with SOCS-7 in a stringent yeast two-hybrid screen of a human leukocyte cDNA-library. We identified the cytoskeletal molecule vinexin as a partner interacting with SOCS-7. Tests with deletion mutants of SOCS-7 demonstrated that a central region of the molecule containing several proline-rich regions, N-terminal to the SH2 domain, was responsible for the binding to vinexin. It is thus likely that one of the SH3 domains of vinexin interacts with a poly-proline region of SOCS-7. The interaction with vinexin was confirmed biochemically as vinexin-alpha was co-precipitated with SOCS-7. Confocal laser-scanning microscopy in HEK293T, MCF-7, and 3T3-L1 cells showed that part of the transfected SOCS-7-green fluorescent protein (GFP) molecules merged with vinexin and with actin. Taken together, our data indicate that SOCS-7 interacts with vinexin and the actin cytoskeleton. |
| |
Keywords: | Actin Vinculin Nck Signal transduction Vinexin |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|