Plant regeneration via somatic embryogenesis in pea (Pisum sativum L.) |
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Authors: | Wilfried Kysely James R Myers Paul A Lazzeri Glenn B Collins Hans-Jorg Jacobsen |
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Institution: | (1) Department of Agronomy, University of Kentucky, 40546-0091 Lexington, KY, USA;(2) Present address: Institut für Genetik, Universität Bonn, Kirschallee 1, D-5300 Bonn 1, Germany;(3) Present address: Research and Extension Center, University of Idaho, 83341 Kimberly, ID, USA;(4) Present address: Max-Planck-Institut, D-5000 Köln 30, Germany |
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Abstract: | Whole plant regeneration via somatic embryogenesis was obtained in pea (Pisum sativum L.) using explants from immature embryos or shoot apex segments. The induction of somatic embryos required picloram or 2,4-D. Germination of fully-developed embryos was accomplished by subculture on medium with only cytokinin and then on medium supplemented with cytokinins in combination with a reduced auxin concentration. Plantlets obtained from both zygotic embryos and shoot apices were transferred to soil and were grown to maturity. Nine plants were examined cytologically, revealing three tetraploids (2n=4x=28) and six diploids (2n=2x=14).Abbreviations Picloram
4-amino-3,5,6-trichloropicolinic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- NAA
1-naphthaleneacetic acid
- BA
6-benzylaminopurine
- IBA
indole-3-butyric acid
KAES Journal Article No. 87-3-4 |
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