Regulation of the synthesis of aryl metabolites by phospholipid sources in the white-rot fungus Bjerkandera adusta |
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Authors: | Carmen Lapadatescu Christian Giniès Aleth Djian Henry-Eric Spinnler Jean-Luc Le Quéré Pascal Bonnarme |
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Institution: | Laboratoire de Recherches sur les Ar?mes, Institut National de la Recherche Agronomique, 17 rue Sully, F-21034 Dijon Cedex, France, FR Laboratoire de Génie et Microbiologie des Procédés Alimentaires (LGMPA), CBAI, Institut National de la Recherche Agronomique, F-78850 Thiverval-Grignon, France e-mail: bonnarme@platon.grignon.inra.fr Tel. +331-30815388; Fax +331-30815597, FR
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Abstract: | The white-rot basidiomycete Bjerkandera adusta was cultivated in a liquid medium enriched with l-phenylalanine and various phospholipid sources (lecithin, egg yolk and asolectin). Three aromatic metabolites (benzaldehyde,
benzyl alcohol and benzoic acid) were produced under these culture conditions. High concentrations of benzaldehyde (404 mg
l–1) were obtained when the cultures were supplemented with 10 g lecithin l–1. Benzyl alcohol production was promoted when the strain was grown with 5 or 10 g lecithin l–1. In the absence of or with a low concentration of lecithin (2.5 g l–1), benzoic acid was the major aryl metabolite synthesized. The results presented here indicate that aryl alcohol oxidase,
an extracellular enzyme catalyzing the oxidation of benzyl alcohol into benzaldehyde, was maximally detected when significant
amounts of benzaldehyde were produced. Aryl alcohol oxidase activity was significantly enhanced in the presence of elevated
concentrations of phospholipid sources. Together with lignin peroxidase, methoxylated and hydroxylated aryl metabolites were
also synthesized under these culture conditions. The possible involvement of phospholipids in the synthesis of aryl metabolites
is discussed.
Received: 7 August 1998 / Accepted: 30 November 1998 |
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Keywords: | Benzaldehyde Benzyl alcohol Benzoic acid Aryl metabolites Bjerkandera adusta Phospholipid sources Fungal metabolism Aryl alcohol oxidase |
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