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Reusability of immobilised Saccharomyces cerevisiae with successive copper adsorption-desorption cycles
Authors:Brendan S. Wilhelm and John R. Duncan
Affiliation:(1) Department of Biochemistry and Microbiology, Rhodes University, P.O Box 94, 6140 Grahamstown, South Africa
Abstract:Summary Immobilised Saccharomyces cerevisiae in batch reactors effectively removed copper from solution with a binding equilibrium of 70 % being attained within 20 minutes of contact. Maximum uptake was between pH 3 to 5 (Vmax = 24.1 mgrmol/g) and was substantially reduced at pH 2. Bound copper was readily recovered by addition of 1.0 M HCl (le % v/v). In adsorption-desorption studies metal removal and recovery was high, and uptake was increased with repeated use. Electron microscopy confirmed that no morphological changes occur to the cells during repeated adsorption-desorption.
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