需氧菌阴道炎、细菌性阴道病阴道菌群分析

用阴道分泌物直接涂片、革兰染色、选择性培养分离和细菌预成酶谱分析等微生物学方法和生物化学方法,分析了正常女性、AV、BV患者的阴道菌群。正常女性阴道中以乳酸杆菌为主,产H2O2乳酸杆菌的检出率89%,AV的致病菌主要是革兰阳性率需氧菌、检出率可达80%,其中金黄色葡萄球菌,粪肠球菌和埃希氏大肠菌较为常见,B族链球菌的检出率较低;BV的致病菌主要是厌氧菌,以革兰阴性厌氧菌的检出率最高,加德纳菌的检出率不足40%。48例BV患者中有8例合并AV感染,31例AV患者中有6例合并BV感染。细菌谱分析发现,AV患者易感染消化链球菌、普雷沃菌等厌氧菌和加德纳菌,BV患者易感染粪肠球菌、大肠埃希菌等需氧菌。

The vaginal micro-flora of aerobic vaginitis and bacterial vaginosis

Objective To analyze and evaluate the vaginal micro-flora in bacterial vaginitis (BV),aerobic vaginitis (AV) and normal female.Method Microbiological and biochemical methods of gram staining, selective culturing and isolating and pre-enzymes profile analysis were used.Result The normal vaginal flora was predominated by lactobacilli, 89% of which were detected as producing H_2O_2.The pathogenic bacteria of AV was primarily Gram-positive aerobic bacteria, with the detection rate of 80% including Staphylococci aueas, Enterococcus faecalis and E.coli;low rate of B. Streptococcic was detected. The pathogenic bacteria of BV was primarily Gram-negative anaerobic bacteria,while the rate of Gardnerella was detected as less than 40%. There were 8 cases with of AV concurrent infection in 48 cases of BV infection,and 6 cases with BV concurrent infection in 31 cases of AV infection. Conclusion Microphyte profile analysis indicated that patients infected by AV were susceptible to anaerobic bacteria such as Gardnerella,Prevost bacteria and Peptostreptococcus,while patients infected BV were susceptible to aerobic bacteria such as Enterococcus faecalis and E.coli etc.