IGS2-RFLP、SCoT和ISSR在白灵侧耳遗传多样性分析中的比较研究

应用IGS2-RFLP、SCoT和ISSR标记分析新疆5个采集地点的28个白灵侧耳野生样本的遗传多样性,比较这3种分子标记在白灵侧耳种质资源遗传多样性研究中的效用。结果显示,IGS2-RFLP的3个内切酶、5个SCoT引物、5个ISSR引物分别检测到42、59和77条多态性条带,多态性比率分别为91.3%、92.4%和92.8%。3种标记检测出的有效等位基因数(ne)和位点平均的预期杂合度(Hep)没有显著性差异。表明3种标记都适宜作为遗传学标记对白灵侧耳野生种质进行多样性分析。多态性检测效率最高的标记为ISSR,E=15.4,Ai=23.4,其次为IGS2-RFLP,E=14.0,Ai=22.4,SCoT则为最低,E=11.8,Ai=18.2。3种标记中,SCoT和ISSR标记的聚类结果极其相似,且均能较为准确地反映样本的地理来源,虽然二者的聚类图不完全相同。IGS2-RFLP的标记效率较高,准确性和可重复性最好,可用于菌株标准图谱的制作,更适用于品种权保护中的菌株鉴定鉴别;SCoT和ISSR标记的多态性高,信息量大,评价范围广,则更适用于白灵侧耳种质资源的遗传多样性研究。

Comparison studies of genetic diversity of Pleurotus eryngii var. tuoliensis by IGS2-RFLP, SCoT and ISSR markers

IGS2-RFLP, SCoT and ISSR markers were applied in the genetic diversity study of 28 wild samples of Pleurotus eryngii var. tuoliensis collected from five locations in Xinjiang Autonomous Region. Effectiveness of the three markers was also compared for genetic diversity evaluation of P. eryngii var. tuoliensis. There were 42, 59 and 77 polymorphic bands from three IGS2-RFLP restriction endonucleases, five SCoT primers and five ISSR primers respectively, with the polymorphism rate of 91.3%, 92.4% and 92.8% separately. The three markers had no significant differences on the effective number of alleles and the average expected heterozygosity of the loci, which indicated that they were all efficient in analysis of the genetic diversity for P. eryngii var. tuoliensis. ISSR marker showed the highest assay efficiency (E=15.4, Ai=23.4), IGS2-RFLP marker was secondly (E=14.0, Ai=22.4), and SCoT marker was the lowest one (E=11.8, Ai=18.2). SCoT and ISSR analysis showed highly similar cluster and could reflect the geographical distribution of the samples more accurately than IGS2-RFLP. IGS2-RFLP marker had the higher assay efficiency and the best repeatability and accuracy, which could be used as a standard fingerprint for strain identification. SCoT and ISSR markers bring a rich in polymorphism and informativeness and they would be applied more efficiently than IGS2-RFLP to germplasm analysis of the genetic diversity for P. eryngii var. tuoliensis.