Identification and characterization of a dense cluster of placenta-specific cysteine peptidase genes and related genes on mouse chromosome 13 |
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| Authors: | Deussing Jan Kouadio Martin Rehman Salima Werber Ingrid Schwinde Anne Peters Christoph |
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| Institution: | 1. Université Hassan 1er, Laboratoire de Chimie et Modélisation Mathématique, Faculté Polydisciplinaire, BP: 25000 Khouribga, Morocco;2. Université Hassan 1er, Laboratoire de Sciences des Matériaux, des Milieux et de la Modélisation, Faculté Polydisciplinaire, BP: 25000 Khouribga, Morocco;1. School of Nursing, Member Magee-Womens Research Institute, University of Pittsburgh, 3500 Victoria St., 448 Victoria Building, Pittsburgh, PA, 15261, United States;2. Cell Biology Associate Director, Center for Biologic Imaging, University of Pittsburgh, United States;2. Department of Cardiothoracic Surgery, Maastricht University Medical Centre, Maastricht, The Netherlands;3. Department of Pulmonology, GROW-School for Oncology and Developmental Biology, Maastricht University Medical Centre, Maastricht, The Netherlands;4. Department of Internal Medicine IV, University Hospital Aachen, Aachen, Germany;5. Department of Neuroscience, Maastricht University, School of Mental Health and Neuroscience (MHeNS), Maastricht, The Netherlands;3. Robarts Research Institute, University of Western Ontario, London, Ontario N6A 5A5, Canada;4. Departments of Biochemistry, University of Western Ontario, London, Ontario N6A 5A5, Canada;5. Departments of Medical Biophysics, University of Western Ontario, London, Ontario N6A 5A5, Canada;6. Departments of Pathology, University of Western Ontario, London, Ontario N6A 5A5, Canada;12. Departments of Medicine, University of Western Ontario, London, Ontario N6A 5A5, Canada;8. London Health Sciences Centre, London, Ontario N6A 5A5, Canada;1. Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Gwanak-gu, Seoul, Republic of Korea;2. Department of Veterinary Physiology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Republic of Korea;3. Animal Biotechnology Division, National Institute of Animal Science, Wanju-gun, Jeollabuk-do, Korea;1. Centre for Genomics, Jiwaji University, Gwalior 474 011, India;2. Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India;3. Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India;4. Sawai Man Singh Medical College, JLN Marg, Jaipur 302 004, India |
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| Abstract: | Genes encoding novel murine cysteine peptidases of the papain family C1A and related genes were cloned and mapped to mouse chromosome 13, colocalizing with the previously assigned cathepsin J gene. We constructed a <460-kb phage artificial chromosome (PAC) contig and characterized a dense cluster comprising eight C1A cysteine peptidase genes, cathepsins J, M, Q, R, -1, -2, -3, and -6; three pseudogenes of cathepsins M, -1, and -2; and four genes encoding putative cysteine peptidase inhibitors related to the proregion of C1A peptidases (trophoblast-specific proteins alpha and beta and cytotoxic T-lymphocyte-associated proteins 2alpha and -beta). Because of sequence homologies of 61.9-72.0% between cathepsin J and the other seven putative cysteine peptidases of the cluster, these peptidases are classified as "cathepsin J-like". The absence of cathepsin J-like peptidases and related genes from the human genome suggests that the cathepsin J cluster arose by partial and complete gene duplication events after the divergence of primate and rodent lineages. The expression of cathepsin J-like peptidases and related genes in the cluster is restricted to the placenta only. Clustered genes are induced at specific time points, and their expression increases toward the end of gestation. The specific expression pattern and high expression level suggest an essential role of cathepsin J-like peptidases and related genes in formation and development of the murine placenta. |
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