A novel vector element providing multicopy vector integration in Arxula adeninivorans |
| |
Authors: | Steinborn Gerhard Gellissen Gerd Kunze Gotthard |
| |
Institution: | Leibniz Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany. |
| |
Abstract: | An Arxula adeninivorans vector element has been identified that provides multicopy integration in an atrp1 host strain. The element consists of the ATRP1 selection marker fused to a newly generated truncated ALEU2 promoter of 53 bp. In the described example eight copies of an amyA expression vector encoding heterologous alpha-amylase from Bacillus amyloliquefaciens are integrated in the genome of the recombinant strain instead of a single copy observed when using the ATRP1 element with the complete promoter. The high copy number results in strains of superior productivity for a secreted recombinant alpha-amylase. The vector design enables the integration of a small vector fragment that consists of yeast DNA only providing high transformation frequencies and a high mitotic stability. |
| |
Keywords: | Arxula adeninivorans auxotrophic selection marker heterologous gene expression multicopy integration transformation yeast integration-expression cassette |
本文献已被 PubMed 等数据库收录! |