Metabolism of [U-13C]Aspartate by Astroglial Cultures: Nuclear Magnetic Resonance Analysis of the Culture Media |
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| Authors: | Murín Radovan Mohammadi Ghasem Kowtharapu Bhavani S Leibfritz Dieter Hamprecht Bernd |
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| Institution: | (1) Interfaculty Institute for Biochemistry, University of Tuebingen, Hoppe-Seyler-Str. 4, 72076 Tuebingen, Germany;(2) Institute for Organic Chemistry, University of Bremen, Bremen, Germany;(3) Present address: Institute for Pharmacology and Toxicology, University of Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany;(4) Department of Vectorology and Experimental Gene Therapy, Biomedical Research Center, University of Rostock, Rostock, Germany; |
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| Abstract: | In brain the amino acid L-aspartate serves roles as: (1) putative transmitter, (2) protein precursor, (3) donor of atoms for
the biosynthesis of pyrimidine and purine bases, and (4) fuel for energy metabolism. Astrocytes dominate aspartate clearance
in brain, and in culture they take up aspartate and quickly metabolize it. In brain, only astrocytes were shown to express
the enzymes for de novo pyrimidine biosynthesis. To gain more details about the spectrum of metabolites generated from aspartate
and subsequently released by cultured astrocytes a 13C-nuclear magnetic resonance analysis was performed of U-13C]aspartate supplemented incubation media exposed to astroglial cultures. The results show that astrocytes readily metabolize
aspartate and release into their culture media 13C-isotopomers of lactate, glutamine, citrate and alanine. Despite the presence in astroglial cells of two tandem enzymes of
pyrimidine biosynthesis and their mRNAs, pyrimidine nucleotide-related heterocyclic compounds such as dihydroorotate and orotate
could not be detected in the culture media. |
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