| 普通烟草和黄花烟草及体细胞杂种过氧化物酶同工酶等电聚焦电泳 |
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| 引用本文： | 王联清,徐金相,赵世民,刘祚昌,陈家玉. 普通烟草和黄花烟草及体细胞杂种过氧化物酶同工酶等电聚焦电泳[J]. 植物生理与分子生物学学报, 1986, 0(3) |
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| 作者姓名： | 王联清 徐金相 赵世民 刘祚昌 陈家玉 |
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| 作者单位： | 中国科学院遗传研究所(王联清，徐金相，赵世民，刘祚昌)，中国科学院遗传研究所(陈家玉) |
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| 摘 要： | 日本学者长尾照义(1978)用原生质体融合获得普通烟草与黄花烟草的体细胞杂种植株。1980年陈家玉等在国内首先获得普通烟草(Copus Yeusuku No.4)和黄花烟草(Yellow Flower)的体细胞杂种植株。之后,中国农科院烟草所(1981)也得到相同的结果。陈家玉等(1983)对上述杂种进行了形态学、细胞学和同工酶的分析并取得一定的结果。Dlineee等(1975)分析比较了用等电聚焦技术分离的过氧
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| 关 键 词： | 体细胞杂种 过氧化物酶同工酶 等电聚焦 烟草 |
ISOELECTRIC FOCUSING ANALYSIS OF PEROXIDASE ISOZYMES OF N.tabacum,N.rustica AND THEIR SOMATIC HYBRIDS |
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| Wang Lian-qing,Xu Jin-xiang,Zao Shi-ming,Liu Zuo-chang and Cheng Ja-yu. ISOELECTRIC FOCUSING ANALYSIS OF PEROXIDASE ISOZYMES OF N.tabacum,N.rustica AND THEIR SOMATIC HYBRIDS[J]. Journal Of Plant Physiology and Molecular Biology, 1986, 0(3) |
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| Authors: | Wang Lian-qing Xu Jin-xiang Zao Shi-ming Liu Zuo-chang Cheng Ja-yu |
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| Abstract: | By isoelectric focusing in polyacrylamide gel, the band patterns of peroxidase isozymes from N. tabacum, N. rustica and their somatic hybrids were compared. The bands 4 and 6 existed in all three experimental materials, band 5 was unique in N. tabacum, bands 7, 8 and 13 specially existed, in N. rustica. The band patterns. of somatic hybrids were complement to both of their parents. However, a new band 2 appeared in some of somatic hybrids. It implied that the band patterns. of peroxidase isozymes of somatic hybrids were relative to their morphology. |
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| Keywords: | peroxidase isozymes tabacco isoelectric focusing somatic hybrid |
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