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Expression of the INU2 gene for an endoinulinase of Aspergillus ficuum in Saccharomyces cerevisiae |
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| Authors: | Hee-Seo Kim Dong Whan Lee Eun Ja Ryu Tai-Boong Uhm Moon-Sik Yang Jung Bae Kim Keon-Sang Chae |
| Affiliation: | (1) Faculty of Biological Sciences, and, Korea;(2) Institute for Molecular Biology and Genetics Chonbuk National University, Chonbuk, 561-756, Korea;(3) R & D Center, Sam Bae Co., Chonbuk, 570-810, Korea |
| Abstract: | The INU2 gene encoding an endoinulinase of Aspergillus ficuum was expressed by the Kluyveromyces marxianus INU1 promoter in a SUC2-deleted Saccharomyces cerevisiae to produce the endoinulinase preparation free of an exoinulinase and an extracellular invertase in the culture medium. A recombinant yeast strain produced the sufficient amount of the enzyme to make a halo around its colony, when inulin was included in the medium. |
| Keywords: | Aspergillus ficuum endoinulinase Gene: INU2 Kluyveromyces marxianus overexpression Saccharomyces cerevisiae |
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