Rapid Sample Processing for Detection of Food-Borne Pathogens via Cross-Flow Microfiltration |
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| Authors: | Xuan Li Eduardo Ximenes Mary Anne Roshni Amalaradjou Hunter B Vibbert Kirk Foster Jim Jones Xingya Liu Arun K Bhunia Michael R Ladisch |
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| Institution: | Laboratory of Renewable Resources Engineeringa;Department of Agricultural and Biological Engineeringb;Weldon School of Biomedical Engineeringc;Department of Food Scienced;Department of Chemistrye;Department of Comparative Pathobiology,f Purdue University, West Lafayette, Indiana, USA |
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| Abstract: | This paper reports an approach to enable rapid concentration and recovery of bacterial cells from aqueous chicken homogenates as a preanalytical step of detection. This approach includes biochemical pretreatment and prefiltration of food samples and development of an automated cell concentration instrument based on cross-flow microfiltration. A polysulfone hollow-fiber membrane module having a nominal pore size of 0.2 μm constitutes the core of the cell concentration instrument. The aqueous chicken homogenate samples were circulated within the cross-flow system achieving 500- to 1,000-fold concentration of inoculated Salmonella enterica serovar Enteritidis and naturally occurring microbiota with 70% recovery of viable cells as determined by plate counting and quantitative PCR (qPCR) within 35 to 45 min. These steps enabled 10 CFU/ml microorganisms in chicken homogenates or 102 CFU/g chicken to be quantified. Cleaning and sterilizing the instrument and membrane module by stepwise hydraulic and chemical cleaning (sodium hydroxide and ethanol) enabled reuse of the membrane 15 times before replacement. This approach begins to address the critical need for the food industry for detecting food pathogens within 6 h or less. |
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