Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans |
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| Authors: | Iskra Katic Helge Gro?hans |
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| Institution: | Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland |
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| Abstract: | We have achieved targeted heritable genome modification in Caenorhabditis elegans by injecting mRNA of the nuclease Cas9 and Cas9 guide RNAs. This system rapidly creates precise genomic changes, including knockouts and transgene-instructed gene conversion. |
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| Keywords: | genome editing CRISPR Cas9 homologous recombination gene conversion Caenorhabditis elegans |
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