| 地高辛标记探针检测重组人干扰素β_(1b)中DNA残留量 |
| |
| 引用本文： | 刘君,张振龙.地高辛标记探针检测重组人干扰素β_(1b)中DNA残留量[J].微生物学免疫学进展,2007,35(2):19-21. |
| |
| 作者姓名： | 刘君 张振龙 |
| |
| 作者单位： | 北京生物制品研究所,北京,100024 |
| |
| 摘 要： | 为检测注射用重组人干扰素β1b半成品中外源性DNA残留量,以重组人干扰素β1b工程菌基因组DNA为模板,用地高辛标记探针,并以此探针进行点杂交。结果证明,该方法检测灵敏度较好,特异性较强,操作较安全简便,可用于重组人干扰素β1b制备过程中的质量监控及半成品的检定。
|
| 关 键 词： | 地高辛 探针 DNA残留量 重组人干扰素β1b |
| 文章编号： | 1005-5673（2007）02-0019-03 |
| 收稿时间： | 2006-12-07 |
| 修稿时间： | 2006-12-072007-01-19 |
Analysis for residual DNA in recombinant human interferon beta 1b using Digoxin labeled probe |
| |
| LIU Jun,ZHANG Zhen-long.Analysis for residual DNA in recombinant human interferon beta 1b using Digoxin labeled probe[J].Progress In Microbiology and Immunology,2007,35(2):19-21. |
| |
| Authors: | LIU Jun ZHANG Zhen-long |
| |
| Abstract: | Genome DNA of engineering bacteria of recombinant human interferon beta 1b were labeled by Digoxin and used as probe for dot hybridization to detect residual DNA in semi-finished products of recombinant human interferon beta 1b for injection.The experimental results showed that specificity and sensitivity of this method are perfect,at the same time,the operation is safe and convenient.This method can be useful for quality control in preparation of recombinant human interferon beta 1b and standardization for semi-finished products. |
| |
| Keywords: | Digoxin Probe Residual DNA Recombinant human interferon beta 1b |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
