Enhancement of somatic embryogenesis frequency by gibberellic acid in fennel |
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Authors: | Gérard Hunault Abdelaziz Maatar |
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Institution: | (1) Station de Biologie végétale A. de Richelieu, Université P. & M. Curie, 72400 Cherré, France |
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Abstract: | The effect of GA3 on somatic embryogenesis from petiole fragments excised from micropropagated fennel plantlets was studied. Explants were maintained for 4 weeks on an induction medium containing, 2,4-d and kinetin and were then transferred to a medium devoid of these growth regulators to allow embryo development. The addition of autoclaved or filter-sterilized GA3 to the induction medium or to the embryo development medium increased the number of embryogenic explants. No positive effect was observed when GA3 was added to the micropropagation medium of the mother plantlets. Gibberellic acid also counteracted the inhibiting effect of continuous light on the number of embryogenic explants. Moreover, the embryogenic frequency of petiole explants from several genotypes previously considered as poorly reacting was highly enhanced by GA3.Abbreviations BA
6-benzyladenine
- 2,4-d
2,4-dichlorophenoxyacetic acid
- GA3
Gibberellic acid
- IAA
Indole-3-acetic acid
- IBA
Indole-3-butyric acid |
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Keywords: | Foeniculum vulgare GA3 genotype effect light-induced inhibition |
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