New approach to model effects of darkness, melatonin and serotonin on Tetrahymena thermophila growth and production of hydrolytic enzymes |
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| Authors: | B. Leclercq J.M. Exbrayat F. Duyme J. De Coninck |
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| Affiliation: | (1) Laboratoires de Microbiologie des Fermentations, Institut Supérieur d'Agriculture, 41 rue du Port, 59046 Lille Cedex, France;(2) Laboratoire de Biologie Générale, Université Catholique de Lyon, 25, rue du Plat, 69288 Lyon Cedex 02, France;(3) d'Informatique et de Statistique, Institut Supérieur d'Agriculture, 41 rue du Port, 59046 Lille Cedex, France |
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| Abstract: | Central composite design was used to quantify the relationship between darkness, melatonin and serotonin with growth ofTetrahymena thermophila and hydrolytic enzyme activities. Circadian variation was the most important factor to optimise the maximal population without a long generation time; i.e. light at the beginning of culture for 640 min followed by darkness for 800 min. In addition, the medium should be supplemented with serotonin and melatonin (0.1 mM each). Supplemental melatonin and serotonin increased lipase, phosphatase and protease activities but without a circadian variation. |
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| Keywords: | darkness melatonin metabolism serotonin Tetrahymena |
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