Serum immunoassay of a small cell lung cancer associated ganglioside: development of a sensitive scintillation proximity assay |
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| Authors: | Tina Pallesen Annette Vangsted Lars Drivsholm Henrik Clausen Jesper Zeuthen Håkan Wallin |
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| Affiliation: | (1) Department of Tumor Cell Biology, The Fibiger Institute, Copenhagen, Denmark;(2) Department of Oncology, State University Hospital, Copenhagen, Denmark;(3) School of Dentistry, University of Copenhagen, Copenhagen, Denmark;(4) Department of Environmental Carcinogenesis, Danish Cancer Society, Copenhagen, Denmark;(5) The Fibiger Institute, The Danish Cancer Society, Ndr. Frihavnsgade 70, DK-2100 Copenhagen, Denmark |
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| Abstract: | We here report an enzyme linked immunosorbent assay (ELISA) and a scintillation proximity assay (SPA) for detection of the ganglioside FucGM1 in sera from small cell lung cancer (SCLC) patients. The SPA was more sensitive and reproducible than the ELISA. In this assay, monoclonal antibodies specific for FucGM1 were bound to SPA particles and incubated with labelled FucGM1 and 100 µl test-serum overnight, and counted in a  -counter. The sensitivity was 0.2 ng. Seven out of twenty sera from SCLC patients were positive, whereas none of twenty sera from healthy individuals were positive for FucGM1. The SPA was more sensitive than the previously reported HPTLC as well as a direct ELISA.Abbreviations MAb monoclonal antibody - SPA scintillation proximity assay - HPTLC high performance thin layer chromatography - SCLC small cell lung cancer - FucGM1 Fuc 1-2Gal1-3GalNAc1-4(NeuAc2-3)-Gal1-4Glc1-1Cer - ELISA enzyme linked immunosorbent assay - FCS foetal calf serum - PBS phosphate buffered saline |
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| Keywords: | SCLC FucGM1 sera immunoassay ELISA scintillation proximity assay |
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