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Endogenous Levels of Gibberellins, IAA and Cytokinins in Tobacco Crown Gall Tissues of Different Morphologies |
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| Authors: | Nakagawa Sachiko; Tjokrokusumo Donowati S; Sakurai Akira; Yamaguchi Isomaro; Takahashi Nobutaka; Syono Kunihiko |
| Institution: | 1The Institute of Physical and Chemical Research Wako-shi, Saitama 351-01, Japan 2Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan 3Department of Pure and Applied Sciences, The University of Tokyo Meguro-ku, Tokyo 153, Japan |
| Abstract: | Endogenous levels of gibberellin (GA) as well as IAA and cytokininsin teratomas and unorganized crown gall tissues of tobacco wereexamined by GC-SIM (for GA and cytokinin) or HPLC with a fluorescencedetector (for IAA). Two different types of crown gall inducedby octopine-type and nopaline-type Ti-plasmids were used. Inboth types, GA contents were higher in shoot-forming teratomasthan in unorganized calluses, while IAA contents were higherin unorganized calluses. But cytokinin contents in octopine-typecells were higher in unorganized calluses than in teratomas,whereas the contents in nopaline-type cells were higher in teratomas.Our results suggest that there is not always a relationshipbetween the cytokinin/IAA balance and tobacco crown gall morphology,but GA production in tobacco tissues is closely related to itsdifferentiation. 4 Present address: Agency for the Assessment and Applicationof Technology (BPPT), Jakarta Pusat, Indonesia. (Received September 1, 1986; Accepted February 16, 1987) |
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