Extended map for the phaseolin linkage group ofPhaseolus vulgaris L |
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Authors: | C. E. Vallejos C. D. Chase |
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Affiliation: | (1) Department of Vegetable Crops, Institute of Food and Agricultural Science, 1255 Fifield Hall, University of Florida, 32611 Gainesville, FL, USA |
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Abstract: | Summary The linkage relationship of 11 bean (Phaseolus vulgaris) seed proteins (including phaseolin), 9 enzyme loci, and theP locus were analyzed in backcross and F2 progenies by use of the software package Mapmaker. The progenies were obtained by crossing the breeding line XR-235-1 and the cultivar Calima. Allelic differences for seed protein loci were detected with SDS-PAGE and those for enzyme loci with starch gel electrophoresis and activity stains. The seed coat color of Calima is a red/beige mottled pattern and that of XR-235-1 is white. Segregation at theP locus was followed by recording the phenotype of the BC1S1 and F3 seed. A linkage group comprising ca. 90 cM was detected with the following gene order:Est-2 — 11 —Pha — 8 — (Spe/Spg) — 24 — P — 9 — (Spa/Spv) — 16 —Spba — 22 —Mdh-1. In addition, another linkage group was detected: (Spd/Spf/Sph) — 5 -Spca. Therefore, the seed proteins appear to be organized in clusters in the bean genome.Florida Agricultural Experiment Station, Journal Series No. R-01131 |
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Keywords: | Common bean Seed proteins Isozymes Mapmaker |
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