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Aqueous two-phase fractionation of biological suspensions for protein recovery from bovine blood |
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| Authors: | Marco Rito-Palomares Christopher Dale Andrew Lyddiatt |
| Affiliation: | (1) Centro de Biotecnología, (I.T.E.S.M.), Campus Monterrey., Suc., México;(2) Smithkline Beechman, 905 Clopper Road, A3, Gaithersburg, U.S.A;(3) Biochemical Recovery Group, Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, UK |
| Abstract: | The practical application of a two-stage aqueous two-phase systems (ATPS) for the fractionation and recovery of proteins from biological suspensions is described. A model process for the recovery of proteins from whole bovine blood was selected to study the implementation of an ATPS process. Recycling of used PEG into the initial extraction stage did not significantly influence the partition behaviour of serum albumin in subsequent cycles. © Rapid Science Ltd. 1998 |
| Keywords: | aqueous two-phase systems protein recovery polymer recycling bovine blood. |
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