Biocatalysed synthesis of sn-1,3-diacylglycerol oil from extra virgin olive oil |
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Authors: | F Blasi L Cossignani MS Simonetti P Damiani |
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Institution: | aSection of Food Chemistry and Analysis, Department of Economic Science and Food Sciences, University of Perugia, Via S. Costanzo, 06126 Perugia, Italy |
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Abstract: | Enzymatic synthesis of sn-1,3-diacylglycerols (sn-1,3-DAG) in two steps without isolation of the intermediates was investigated. Firstly ethanolysis of extra virgin olive oil (EVO) using immobilized non-regiospecific lipase from Candida antarctica (Novozym 435) was carried out to obtain glycerol (Gly) and fatty acid ethyl esters (FAEE). In the second step the ethanolysis products have been re-esterificated testing different sn-1,3-regiospecific lipases, both immobilized and non-immobilized, in different reaction media, that is in the presence of solvents or in a solvent-free system, for different times, at different temperatures (12, 25 and 40 °C). The lipase from Rhizomucor miehei (Lipozyme IM) has been the most effective among the sn-1,3-specific lipases screened. |
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Keywords: | sn-1 3-Diacylglycerols Extra virgin olive oil Novozym 435 Lipozyme IM |
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