Chromosome replication in Escherichia coli induced by oversupply of DnaA |
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Authors: | Y -C Xu and H Bremer |
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Institution: | (1) Biology Programs, The University of Texas at Dallas, P.O. Box 688, 75080 Richardson, TX, USA |
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Abstract: | Summary Overexpression of DnaA protein from a multicopy plasmid accompanied by a shift to 42°C causes initiation of one extra round of replication in a dnaA
+ strain grown in glycerol minimal medium. This extra round of replication does not lead to an extra cell division, such that cells contain twice the normal number of chromosomes. |
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Keywords: | Escherichia coli DnaA Replication initiation |
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