Effects of beta-bungarotoxin on calcium uptake by sarcoplasmic reticulum from rabbit skeletal muscle |
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| Authors: | Y H Lau T H Chiu A H Caswell |
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| Institution: | Department of Pharmacology, University of Miami School of Medicine Miami, Florida 33152 USA |
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| Abstract: | A fluorescent chelate probe and a Millipore filtration technique have been used to study the effects of β-bungarotoxin (β-toxin) on passive and active Ca++ uptake and ATPase in fragmented sarcoplasmic reticulum (SR) of rabbit skeletal muscle. β-Toxin at 3 × 10?6 M did not affect ATPase activity. In the absence of ATP, β-Toxin increased the passive uptake of Ca++; in the presence of ATP, active Ca++ uptake was inhibited. The effect of β-toxin in SR can be detected at concentrations as low as 10?9 M. The results suggest that β-toxin induces Ca++ leakage in SR membranes. |
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