Quantitative measurement of cell-bubble attachment in bubble columns using foam flotation techniques |
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Authors: | Zhi-You Wen Wen-Song Tan |
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Institution: | (1) Department of Botany, the University of Hong Kong, Pokfulam Road, Hong Kong;(2) State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai , 200237, P.R. China |
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Abstract: | A simple and convenient system for quantitatively measuring the number of adsorbed animal cells per unit of bubble surface area ( , unit: cells/cm2) was developed. The system was successfully applied to recombinant Chinese hamster ovary (r-CHO) suspension cultures to investigate the dynamic cell-bubble attachment in a bubble column. In serum-free medium, values increased with bubble rising height (H) and cell concentration (C) and then became constant (about 1750 cells/cm2) when H and C were sufficiently high. In medium containing protective additives, the trends of values with H were similar to that in serum-free medium. Compared with serum-free medium, polyvinyl-pyrrolidone (PVP) increased the values to 1941 cell/cm2 whereas other tested additives decreased the values of in some different degree. |
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Keywords: | animal cell culture cell-bubble interaction foam flotation protective additives bubble column |
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