Production of hybrids, amphiploids and backcross progenies between a cold-tolerant wild species, Erucastrum abyssinicum and crop brassicas |
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Authors: | G U Rao M Lakshmikumaran K R Shivanna |
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Institution: | (1) Department of Botany, University of Delhi, 110 007 Delhi, India;(2) Tata Energy Research Institute, Darbari Seth Block, Habitat Place, 110 003 New Delhi, India |
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Abstract: | Three intergeneric hybrids were produced between a cold-tolerant wild species, Erucastrum abyssinicum and three cultivated species of Brassica, B. juncea, B. carinata and B. oleracea, through ovary culture. The hybrids were characterized by morphology, cytology and DNA analysis. Amphiploidy was induced in all the F1 hybrids through colchicine treatment. Stable amphiploids and backcross progenies were obtained from two of the crosses, E. abyssinicum x B. juncea and E. abyssinicum x B. carinata. The amphiploid, E. abyssinicum x B. juncea was successfully used as a bridge species to produce hybrids with B. napus, B. campestris and B. nigra. These hybrids and backcross progenies provide useful genetic variability for the improvement of crop brassicas. |
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Keywords: | Erucastrum abyssinicum Brassica spp Intergeneric hybrids Bridge crosses Amphiploidy Stress resistance |
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