The optimization of serum-free medium for the production of the scu-PA by the addition of algal extracts |
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Authors: | Kim H G Sung K D Ham M S Chung K H Chung K H Lee H Y |
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Institution: | (1) Department of Food Engineering, Kangwon National University, 200-701 Chunchon, S. Korea;(2) Mokam Biotechnology Institute Seoul, S. Korea |
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Abstract: | The addition of 2.8 g/ml algal extracts enhanced both scu-PA production and cell growth in a serum-free medium, compared to a conventional serum-free medium for the cultivation of recombinant CHO cells. The growth rate and scu-PA production were relatively lower in the serum-free medium than 5% serum containing medium: however, specific scu-PA production rate was higher in the serum-free medium due to the long-term period of cultivation (3.66×10–4 vs. 2.48×10–4 IU/cell/day). Overall scu-PA production rate was also greater in an enforced serum-free medium as 25,000 IU/day over 50 d of perfusion cultivation. The conversion ratio of scu-PA to tcu-PA was greatly reduced in the serum-free medium during perfusion cultivation (10% compared to 20% conversion in a serum containing medium). |
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Keywords: | Serum-free medium scu-PA production algal extracts |
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