Partition of isomeric dipeptides in poly(ethylene glycol)/magnesium sulfate aqueous two-phase systems |
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Authors: | M A Eiteman J L Gainer |
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Affiliation: | Department of Chemical Engineering, University of Virginia, Charlottesville 22903-2442. |
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Abstract: | A series of isomeric dipeptides, i.e., those containing identical residues but in different order such as Trp-Gly versus Gly-Trp, was partitioned in a poly(ethylene glycol) (PEG)/magnesium sulfate (MgSO4) aqueous two-phase system. Dipeptides having a more hydrophobic character favored the upper (PEG) phase. Moreover, the partition coefficients for isomeric dipeptides are different, with the partition coefficients for dipeptides containing the more hydrophobic residue in the C-terminal position being, in general, greater than the partition coefficients for corresponding isomers which contain the more hydrophobic residue in the N-terminal position. These observations can be attributed to the different interactions that the isomers have with specific two-phase systems. |
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