|
|||||
|
|
A new xylanase from a Trichoderma harzianum strain |
|
| Authors: | F Q de Paula Silveira M V de Sousa C A O Ricart A M F Milagres C L de Medeiros E X F Filho |
| Affiliation: | Laboratório de Enzimologia, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, CEP 70910-900, Brazil, BR CBSP-Centro Brasileiro de Servi?os e Pesquisas em Proteínas, Departmento de Biologia Celular, Universidade de Brasília, DF, CEP 70910-900, Brazil, BR Faculdade de Engenharia Química de Lorena, Departamento de Biotecnologia, Caixa Postal 116, Lorena, SP, CEP 12600-000, Brazil, BR |
| Abstract: | A new xylanase (XYL2) was purified from solid-state cultures of Trichoderma harzianum strain C by ultrafiltration and gel filtration. SDS-PAGE of the xylanase showed an apparent homogeneity and molecular weight of 18 kDa. It had the highest activity at pH 5.0 and 45°C and was stable at 50°C and pH 5.0 up to 4 h xylanase. XYL2 had a low K m with insoluble oat spelt xylan as substrate. Compared to the amino acid composition of xylanases from Trichoderma spp, xylanase XYL2 presented a high content of glutamate/glutamine, phenylalanine and cysteine, and a low content of serine. Xylanase XYL2 improved the delignification and selectivity of unbleached hardwood kraft pulp. Received 02 February 1999/ Accepted in revised form 17 April 1999 |
| Keywords: | : Trichoderma xylan xylanase characterization |
| 本文献已被 SpringerLink 等数据库收录！ | |