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Optimization of parental RNAi conditions for hunchback gene in Locusta migratoria manilensis (Meyen) |
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| Authors: | Zheng-Bo He Yue-Qing Cao Yu-Xian Xia Genetic Engineering Research Center College of Bioengineering Chongqing University Chongqing Engineering Research Center for Fungal Insecticides Key Laboratory of Functional Gene Regulation Technologies under Chongqing Municipal Education Commission Chongqing China Institute of Insect Molecular Biology College of Life Sciences Chongqing Normal University Chongqing China |
| Institution: | Zheng-Bo He,Yue-Qing Cao and Yu-Xian Xia Genetic Engineering Research Center,College of Bioengineering,Chongqing University,Chongqing Engineering Research Center for Fungal Insecticides and Key Laboratory of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission,Chongqing,China Institute of Insect and Molecular Biology,College of Life Sciences,Chongqing Normal University,Chongqing 400047,China |
| Abstract: | An investigation on the optimization of parental RNA interference (RNAi) conditions for hunchback {hb) gene in Locusta migratoria manilensis (Meyen) was conducted. Double stranded RNA (dsRNA) corresponding to hb gene was injected into haemocoel of female adults of L.migratoria manilensis.Embryos developed from the eggs laid by the injected adults on the 7th day after eclosion showed observable effects of RNAi for hb.The silencing effect after delivery treatment of dsRNA for hb gene was maintained for more t... |
| Keywords: | hunchback Locusta migratoria manilensis parental RNA interference |
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