Removal of zero-quantum interference in NOESY spectra of proteins by utilizing the natural inhomogeneity of the radiofrequency field |
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| Authors: | L. Mitschang J. Keeler A. L. Davis H. Oschkinat |
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| Affiliation: | (1) European Molecular Biology Laboratory, Meyerhofstrasse 1, 6900 Heidelberg, Germany;(2) Department of Chemistry, University of Cambridge, Lensfield Road, CB2 1EW Cambridge, UK |
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| Abstract: | Summary A single scan method for the suppression of signals arising from zero-quantum coherences (ZQC) is analysed with respect to its application to NMR experiments on proteins. The ZQC are dephased during a spinlock period due to the natural RF inhomogeneity of a commercial probe. A quantitative analysis of a ZQC-compensated NOESY experiment is given. Although the build-up curve for the cross peaks in ZQC-compensated NOESY experiments differ from those in uncompensated experiments, interproton distances in medium-sized proteins can be evaluated with high accuracy. The proposed method is compared with other techniques for ZQC suppression. |
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| Keywords: | Zero-quantum coherence Inhomogeneity of RF field Cross relaxation Interproton distances Proteins |
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