| 氮源及碳氮比对产朊假丝酵母合成谷胱甘肽的影响 |
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| 引用本文： | 聂敏,卫功元,邵娜,葛晓光. 氮源及碳氮比对产朊假丝酵母合成谷胱甘肽的影响[J]. 生物加工过程, 2010, 8(2): 23-29. DOI: 10.3969/j.issn.1672-3678.2010.02.005 |
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| 作者姓名： | 聂敏 卫功元 邵娜 葛晓光 |
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| 作者单位： | 苏州大学,医学部基础医学与生物科学学院,苏州,215123 |
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| 基金项目： | 苏州大学医学部青年基金 |
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| 摘 要： | 研究了N源对产朊假丝酵母细胞生长和谷胱甘肽(GSH)合成的影响。在此基础上,分别以(NH4)2SO4和尿素作为单一N源,摇瓶条件下研究了不同C、N比对GSH发酵的影响。结果发现尿素有利于细胞生长,而(NH4)2SO4更有利于GSH的合成,并且酵母细胞在利用这2种N源合成GSH时,各自具有最佳的C、N比((NH4)2SO4为8.3 mol/mol,尿素为5.6 mol/mol)。最佳C、N比下的GSH分批发酵结果显示,尿素是更合适的N源,最终细胞干质量和GSH产量可以分别达到16.48 g/L和246.4 mg/L。最后分别采用发酵动力学模型和代谢网络分析对该结果产生的原因进行了定量解释。
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| 关 键 词： | 分批发酵 产朊假丝酵母 碳氮比 谷胱甘肽 |
Effect of nitrogen source and C/N ratio on the fermentative production of glutathione by Candida utilis |
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| NIE Min,WEI Gong-yuan,SHAO Na,GE Xiao-guang. Effect of nitrogen source and C/N ratio on the fermentative production of glutathione by Candida utilis[J]. Chinese Journal of Bioprocess Engineering, 2010, 8(2): 23-29. DOI: 10.3969/j.issn.1672-3678.2010.02.005 |
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| Authors: | NIE Min WEI Gong-yuan SHAO Na GE Xiao-guang |
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| Affiliation: | NIE Min,WEI Gong-yuan,SHAO Na,GE Xiao-guang(School of Basic Medicine , Biological Science , College of Medicine,Soochow University,Suzhou 215123,China) |
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| Abstract: | The effect of nitrogen sources on cell growth and GSH production by Candida utilis in shaking flasks was studied.(NH4)2SO4 and urea were individually chosen as the single nitrogen source at varied C to N ratio.Urea was beneficial to cell growth while(NH4)2SO4 was favorable to GSH production.The optimal C/N molar ratio were 8.3 and 5.6 for(NH4)2SO4 and urea.Batch fermentation of GSH using the single nitrogen source was conducted under the optimal C/N ratios.The maximum dry cell weight and GSH concentration o... |
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| Keywords: | batch fermentation Candida utilis carbon nitrogen ratio glutathione |
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