| 米根霉利用纯糖和不同预处理玉米秸秆酶解糖生产L-乳酸 |
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| 引用本文： | 李鑫,马瑞,赵晗璐,欧阳嘉,徐勇,勇强.米根霉利用纯糖和不同预处理玉米秸秆酶解糖生产L-乳酸[J].生物加工过程,2010,8(6):1-5. |
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| 作者姓名： | 李鑫 马瑞 赵晗璐 欧阳嘉 徐勇 勇强 |
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| 作者单位： | 南京林业大学,林木遗传与生物技术省部共建教育部重点实验室,南京,210037 |
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| 基金项目： | 国家高技术研究发展计划(863计划)资助项目，国家重点基础研究发展计划(973计划)资助项目，南京林业大学高学历人才基金项目 |
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| 摘 要： | 通过单因素实验设计,优化米根霉摇瓶发酵产L-乳酸。在此基础上,以蒸气爆破和碱处理玉米秸秆酶解液为混合C源,与纯糖对比,研究不同预处理玉米秸秆混合C源对米根霉发酵产L-乳酸的影响。结果显示:在初始葡萄糖质量浓度100g/L、(NH4)2SO4质量浓度2g/L、接种量6%(体积分数)、转速170r/min、发酵12h后添加30g/LCaCO3的条件下,米根霉发酵产L-乳酸质量浓度为69.15g/L。米根霉发酵不同预处理玉米秸秆酶解混合C源,木糖的存在影响了米根霉的C代谢网络,降低L乳酸的产量。
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| 关 键 词： | 玉米秸秆 水解物 L-乳酸 米根霉 木糖 |
L-lactic acid produced from carbohydrate and enzymatic hydrolyzate of different pretreated corn stovers by Rhizopus oryzae |
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| LI Xin,MA Rui,ZHAO Han-lu,OUYANG Jia,XU Yong,YONG Qiang.L-lactic acid produced from carbohydrate and enzymatic hydrolyzate of different pretreated corn stovers by Rhizopus oryzae[J].Chinese Journal of Bioprocess Engineering,2010,8(6):1-5. |
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| Authors: | LI Xin MA Rui ZHAO Han-lu OUYANG Jia XU Yong YONG Qiang |
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| Institution: | LI Xin,MA Rui,ZHAO Han-lu,OUYANG Jia,XU Yong,YONG Qiang(Key Laboratory of Forest Genetics & Biotechnology of the Ministry of Education,Nanjing Forestry University,Nanjing 210037,China) |
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| Abstract: | Fermentation conditions for production of L-lactic acid by Rhizopus oryzae were optimized in single-factor experiments.Compared with carbohydrates,the production of L-lactic acid was investigated using the enzymatic hydrolyzate(containing glucose and xylose)from steam pretreated and alkali pretreated corn stovers.The optimal culture conditions by single-factor design were as follows:initial glucose 100 g/L,(NH4)2SO4 2 g/L,inoculum 6%(v/v),170 r/min,and adding 30 g/L CaCO3 after 12 h fermentation.The concent... |
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| Keywords: | corn stover hydrolyzate L-lactic acid Rhizopus oryzae xylose |
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