Removal of a bound ligand from a macromolecule by gel filtration. |
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Authors: | H B Dixion |
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Abstract: | If a solution of macromolecule of concentration c and volume a, initially saturated with a ligand of dissociation constant K, is gel filtered through a volume v of solution initially free of ligand, the saturation y with which it emerges is given by the equation y-lny-1=Kv/ca. |
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