Delayed methylation and the matrix bound DNA methylase |
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| Authors: | T Davis D Kirk A Rinaldi R H Burdon R L Adams |
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| Affiliation: | 1. Institute of Human Genetics, CNRS UPR1142, Molecular Basis of Cancer-Related Inflammation Laboratory, University of Montpellier, Montpellier, France;2. Institute of Human Genetics, CNRS UPR1142, Molecular Virology Laboratory, University of Montpellier, Montpellier, France;1. Savannah River Ecology Laboratory, Aiken, SC 29802, USA;2. Eugene P. Odum School of Ecology, University of Georgia, Athens, GA 30602, USA;1. Department of Mathematics, School of Science and Engineering, University of Chester, Thornton Science Park, Chester CH2 4NU, UK;2. Department of Epidemiology and Biostatistics, School of Public Health, Imperial College London, St Mary''s Campus, London W2 1PG, UK;3. Department of Chemical Engineering, School of Science and Engineering, University of Chester, Thornton Science Park, Chester CH2 4NU, UK |
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| Abstract: | It is shown that the methylation of DNA that occurs in isolated nuclei is "delayed methylation". This methylation is not reduced in nuclei which have been pretreated with 0.2M NaCl to extract the soluble methylase suggesting that this methylation is the product of a firmly bound matrix associated DNA methylase. Evidence is provided that, like the methylase, the DNA substrate is associated with the nuclear matrix. |
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