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Gene note. Cloning of an Arabidopsis cDNA encoding a chloroplast localizing sulphate transporter isoform
Authors:Takahashi  H; Asanuma  W; Saito  K
Institution:Laboratory of Molecular Biology and Biotechnology, Research Center of Medicinal Resources, Faculty of Pharmaceutical Sciences, Chiba University, Yayoi-cho 1-33, Inage-ku, Chiba 263-8522, Japan; Corresponding author; Fax: +81 43 290 2905; E-mail: ksaito@p.chiba-u.ac.jp
Abstract:A new member of the sulphate transporter gene in Arabidopsis encoding a chloroplast-localizing isoform, AST82 (accession no. AB008782) has been identified. AST82 had a sequence similarity with a cyanobacterial genome sequence, slr1776, which encodes a putative eukaryotic proton/sulphate co-transporter. Fusion proteins of AST82 and green fluorescent protein (GFP) transiently expressed in Arabidopsis leaves localized in chloroplasts, indicating that AST82 encodes a chloroplastic isoform.
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