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Microscopy of living epithelial cells upon the intact ocular lens in culture
Authors:J F Gierthy  S N Borrow  H Rothstein
Affiliation:1. Department of Zoology, University of Vermont, Burlington, Vt 05401, USA;2. Downstate Medical Center, State University of New York, Brooklyn, N.Y. 11203, USA;1. Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China;2. Ying Tan People''s Hospital, Yingtan, China;3. Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, China;4. Tong Ren People''s Hospital, Chongqing, China;5. Chongqing Xinqiao Community Health Service Center, Chongqing, China;1. Agrosup Dijon, 26 boulevard Docteur Petitjean, BP 87999, 21079 Dijon Cedex, France;2. Laboratoire Electronique, Informatique et Image, University of Burgundy, BP 47870, 21078 Dijon Cedex, France;1. Department of Viral Vaccines, Chengdu Institute of Biological Products Co., Ltd, China National Biotech Group, Chengdu 610023, PR China;2. Department of Arbovirus Vaccines, National Institutes for Food and Drug Control, Beijing 100050, PR China
Abstract:Epithelial cells on cultured lenses have been observed in the living state, for the first time, by both phase contrast and interference-contrast microscopy. Our observations show that within 48 h after explantation the cells undergo a transformation in shape from polygons to spindles. By 72–87 h post-isolation the spindle shaped cells round up to varying degrees and many of them undergo mitosis.
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