Survival of several <Emphasis Type="Italic">Rhizobium/Bradyrhizobium</Emphasis> strains on different inoculant formulations and inoculated seeds |
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Authors: | F Temprano M Albareda M Camacho A Daza C Santamaría Nombre D Rodríguez-Navarro |
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Institution: | (1) CIFA "Las Torres y Tomejil", Apartado Oficial, 41200 Alcalá del Río, Sevilla, Spain, |
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Abstract: | The effect of a variety factors on the survival of several rhizobia strains on inoculants and inoculated seeds has been evaluated.
Since the rhizobia strains showed different cell-density-evolution patterns on peat-based inoculants and on inoculated seeds,
several inoculant formulations with highly effective Rhizobium/Bradyrhizobium strains (for Lupinus, Hedysarum, Phaseolus and Glycine max.) were monitored under the following storage conditions: (a) the inoculants were kept refrigerated (at 4 °C), or (b) at room
temperature (25 °C). The effect of water content (30–50%, w/w) in the inoculants as well as that of several seed-coating adhesives
were also investigated. Alternative carriers including perlite and vermiculite were tested. For all of the strains, survival
on sterile peat-based inoculants was higher than on the corresponding unsterile peat formulation; for the latter, refrigerated
storage conditions are recommended to ensure high bacterial densities. The water content of the inoculants had a differential
effect on strain survival depending on the sterility of the peat, such that a high water content was more detrimental when
unsterilized peat was employed. The best adherent for rhizobia survival was a gum arabic/water solution. Perlite was as effective
as peat in maintaining a high population of rhizobia, at least for 6 months of storage.
Electronic Publication |
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Keywords: | Rhizobium spp Bradyrhizobium spp Sinorhizobium fredii Inoculants Carriers |
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